Interdomain loop mutation Asp190Cys of the tetracycline efflux transporter TetA(B) decreases affinity for substrate.
نویسندگان
چکیده
TetA(B), the tetracycline efflux protein from Tn10, has 12 predicted α-helices that span the Escherichia coli cytoplasmic membrane (10) and is a member of the Major Facilitator Superfamily (4). TetA(B) and related tetracycline antiporters use the bacterial transmembrane proton motive force to export one metal-tetracycline complex in exchange for one H + (12), thereby reducing the intracellular tetracycline concentration.
منابع مشابه
Substitutions in the interdomain loop of the Tn10 TetA efflux transporter alter tetracycline resistance and substrate specificity.
Cysteine replacement of Asp190, Glu192 and Ser201 residues in the cytoplasmic interdomain loop of the TetA(B) tetracycline efflux antiporter from Tn10 reduces tetracycline resistance [Tamura, N., Konishi, S., Iwaki, S., Kimura-Someya, T., Nada, S. & Yamaguchi, A. (2001). J Biol Chem 276, 20330-20339]. It was found that these Cys substitutions altered the substrate specificity of TetA(B), increa...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 51 8 شماره
صفحات -
تاریخ انتشار 2007